275P - Immunohistochemical Ascertainment Improves the Prognostic Value of Lymphatic and Blood Vessel Invasion in Primary Ductal Breast Cancer

Abstract

ABSTRACT Aim: Lymphovascular invasion (LBVI) including lymphatic (LV) and blood (BVI) vessel invasion is a critical step in cancer metastasis. In breast cancer, the optimal detection method of LBVI remains unclear. This research aimed to compare the prognostic value of different assessment of the LVI and BVI in patients with early breast cancer. Methods: The study cohort included 360 patients with a median follow-up of 168 months. LBVI on H&E sections (LBVIH&E) was identfied fom reviewing H&E slides. Immunohistohemical stining for D2-40 and Factor VIII was performed to identify LVID2-40 and BVIFVIII. Results: LBVIH&E, LVID2-40 and BVIFVIII were present in 102(28%), 127(35%) and 59(16%) patients respectively. In node negative patinets (206), LBVIH&E, LVID2-40 and BVIFVIII were present in 41(20%), 53(26%) and 21(10%) respectively. In tripe negative patients (150), LBVIH&E, LVID2-40 and BVIFVIII were present in 47(31%), 61(41%) and 21(14%) respectively. LBVIH&E was significantly associated with tumour recurrence in the whole cohort (P Conclusions: Assessment of LVI and BVI by IHC using D2-40 and Factor VIII improves prediction of outcome in patients with node negative and triple negative breast cancer. These results make a case for routine clinical assessment of lymphatic and blood vessel invasion by IHC to ascertain LVI and BVI. Disclosure: All authors have declared no conflicts of interest.