#273 Identification of epipin expression in progression of chronic kidney disease—a multi-omic approach

Abstract

Abstract Background and Aims Chronic kidney disease (CKD) is characterized by irreversible reduction in kidney function eventually leading to end-stage renal disease (ESRD). ESRD is a stage when kidney function is no longer adequate for long-term survival without dialysis or kidney transplant. Over the past the prevalence is increased more than 3 fold. Impaired activity of endogenous renal matrix-degrading proteases enhance interstitial matrix accumulation but specific pathways are involved remain unclear. Hence, an urgent need for sensitive biomarkers can detect disease earlier than traditional one and predict disease progression. Present study intended to identify epipin during chronic kidney disease and also we highlight promising kidney biomarkers that have robust underpinnings in the pathophysiology of kidney damage. Method A cross sectional study was conducted on patients with CKD compared with patients with diabetes mellitus (DM) and healthy controls. The collected samples were analyzed for the biochemical investigations included renal profile tests such as urea, serum creatinine, uric acid and urine spot test was analyzed for the screen of kidney problems. Firstly we identified the novel epipin protein through LC-MS then we estimated its gene expression levels using RT-PCR and levels of protein expression measured by ELISA. Results Epipin was shown higher expression in CKD compared to controls and moderately expressed in patients with diabetes mellitus compared to controls. The expression level of epipin gene was 4 fold in DM compared with controls, 19 fold in CKD compared with controls and 5 fold change between CKD and DM. Epipin showed AUC of 0.99 and 1.00 in DM and CKD respectively. The present study found that epipin was highly expressed in CKD patients and moderately expressed in patients with diabetes mellitus when compared to controls. In a present study, we identified one of the epipin with increased expression in renal disease progression. Conclusion The expression of epipin in virtually all samples suggests that this intracellular protein may have diverse and an extensive biological roles and may be an important therapeutic target for development of renal disease and its progression.