271 In Vitro Study of Anti-Inflammatory Activities for Various Probiotics Strains

Abstract

Objectives and study: The aim of this study is to screen promising probiotics strains according to their in vitro inhibitory effects on pathogen-induced inflammation of intestinal epithelium cells.Methods: Different commercial Lactobacillus strains were investigated. Inflamed differentiated Caco-2 cells were triggered by Salmonella lipopolysaccharide for 48 hours. Lipopolysaccharide was removed after 24 hours of inoculation and different concentrations of Lactobacillus were apically cultured with the inflamed Caco-2 cells for another 24 hours. Immune responses, including chemokine secretion (TNF-alpha and IL8) and gene-expression (RT-PCR) (IL6, IL8 and TGF-beta) were studied at different time points for characterization of changes in Caco-2 cells cocultured with or without lactobacilli.Results: The mRNA expression of various cytokines and IL-8 secretion in lactobacilli-treated Caco-2 cells were lower compared with controls. Lactobacillus acidophilus exerted the obvious anti-inflammatory effect on the Salmonella lipopolysaccharide-stimulated intestinal epithelium. Additionally, most Lactobacillus strains displayed various anti-inflammatory capabilities in different time points and doses. Table Table 1 [Inhibition of TNF-alpha secretion]Full size table Conclusion: The chosen Lactobacillus strains can be good candidates as probiotic therapeutic agents for anti-inflammation. Our in vitro Caco-2 cell culture may provide a reliable and reproducible model for large-scale surveys in the characterization of commercial probiotics.