27 RELATIONSHIP BETWEEN GENETIC POLYMORPHISM OF ANGIOTENSINOGEN M235T GENE AND ESSENTIAL HYPERTENSION AMONG ETHNICS IN SOUTHEAST SULAWESI

Abstract

Background: The studies on genetic polymorphism of angiotensinogen M235T gene conducted in several countries showed that the emergence of allele 235T among Asians had increased the risk of hypertension compared to Caucasians. Nevertheless, relations between angiotensinogen gene polymorphism and essential hypertension risk on intra-populations of asian people between ethnics is still less consistent. Objective: This study aimed to examine the relationship in polymorphism of angiotensinogen M235T gene as a risk factor causing essential hypertension among ethnics in Southeast Sulawesi. Method: This research used cross sectional study design. Sixty-one genetic samples (peripheral venous blood from 53 hypertensive and 8 normotensive subjects) were processed through polymerase chain reaction and restriction fragment length polymerase. Results: Tolaki ethnic who had homozygous TT genotype were 27 samples (44.3% of total sample) and homozygous MT genotype were 6 samples (9.8%). Muna ethnic who had homozygous TT genotype were 22 samples (36.1%) and there were no heterozygous MT genotype. Buton ethnic who had homozygous TT genotype were 5 samples (8.2%) dan heterozygous MT genotype was found in 1 sample (1.6%). Overall, the numbers of homozygous TT genotype were 54 samples (88.5%), homozygous MT genotypes were 7 (11.5%) and no subject was homozygous MM genotype. Among the hypertensive group, 46 were TT genotype and 7 were MT genotype. In the normotensive group (all 8 samples), only TT genotype was found (chi-square 1.94, p-value 0.275). Conclusion: This study concluded that there was no relationship between polymorphism of Angiotensinogen M235T gene and essential hypertension among ethnics in Southeast Sulawesi.