Abstract
Conjugation of steroids with polar groups provides them with increased solubility in most biological fluids. In consequence, steroids are predominantly present in human blood and urine as sulfated compounds. The cycle of sulfonation-desulfonation is classically attributed to two types of enzymes: sulfotransferases (SULTs), which sulfonate hydroxyl groups of the steroids, and steroid sulfatase (STS), which releases the sulfate group to obtain the free active steroid.
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