Abstract
The presence of superoxide, hydrogen peroxide, and a catalytic amount of iron complexed to an appropriate liquid, such as di- or triphosphate nucleotides, will lead to the production of hydroxyl free radicals (OH·). Hydroxyl free radicals are extremely reactive and thus would be present at any specific time in at most very low amounts in biological systems even under conditions of severe oxidative stress. Detection of hydroxyl free radicals per se by electron paramagnetic resonance (EPR) in biological systems is virtually impossible; however, the use of spin-trapping techniques offers possibilities. The results presented illustrate that the hydroxyl-free-radical adduct of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) (DMPO-OH·) and its one-electron reduction product (DMPO-OH 2 ) can be separated out and detected by high-pressure liquid chromatography (HPLC)-electrochemical detection. It is clear, because biological systems tend to reduce DMPO-OH· to the diamagnetic DMPO-OH 2 and because HPLC-electrochemical detection offers extreme sensitivity as well as selective separation that this method has excellent potential of being very useful as a tool for studying oxygen free radicals in biological systems.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
