Read

[27] Applications of Renilla bioluminescence: An introduction

Publication Date Jan 1, 1978

Abstract

Publisher Summary This chapter focuses on the application of Renilla bioluminescence system including the characteristics of the proteins and substrates involved in the light-emitting process. The molecular basis for Renilla bioluminescence, as well as certain features of its nerve-linked control are also described. The requirement for 3´,5´-diphosphoadenosine (PAP) in Renilla bioluminescence involved a cofactor in the conversion of luciferyl sulfate to coelenterate-type luciferin by the enzyme luciferin sulfokinase. The assay for PAP involves a coupled-enzyme luminescence assay utilizing luciferyl sulfate as the substrate. Renilla luciferase is purified to homogeneity from crude extracts with an overall recovery of 24%. Luciferase also contains three free SH groups but no disulfide linkages. Renilla luciferase can be classified as an oxygenase (O 2 ) as it is apparently incorporated into one of the products, oxyluciferin, while a second product, carbon dioxide (CO 2 ) is derived from carbon 3 of luciferin. The initial product of the reaction is an electronic excited state of luciferaseoxyluciferin-monoanion complex that relaxes to its ground state with the production of blue light.

Concepts
Powered ByUnsilo

Renilla Luciferase
Free SH Groups
Disulfide Linkages
Conversion Of Sulfate
Crude Extracts
Production Of Light
Carbon Dioxide
Ground State
Application Of System
Free Groups

Introducing Weekly Round-ups!Beta

Powered by R DiscoveryR Discovery

Round-ups are the summaries of handpicked papers around trending topics published every week. These would enable you to scan through a collection of papers and decide if the paper is relevant to you before actually investing time into reading it.

Climate change Research Articles published between Jun 20, 2022 to Jun 26, 2022

R DiscoveryJun 27, 2022
R DiscoveryArticles Included:  2

One eighth of the bird species in the world is considered globally threatened; the avifauna of Iraq comprises 409 species and is considered as the maj...

Read More

Coronavirus Pandemic

You can also read COVID related content on R COVID-19

R ProductsCOVID-19

ONE PROBLEM . ONE PURPOSE . ONE PLACE

Creating the world’s largest AI-driven & human-curated collection of research, news, expert recommendations and educational resources on COVID-19

COVID-19 Dashboard