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Abstract
Xanthine oxidase has been recognized as an important source of oxygen free radicals in ischemia-reperfusion injury. In order to study enzyme in biological tissues, the conversion of of pterin (2-amino-4-hydroxypteridine) of isoxanthopterin provides the basis for a very sensitive fluorometric assay. Xanthine oxidase is typically assayed in the presence of pterin only, while an electron acceptor which replaces NAD + is used to determine the combined xanthine dehydrogenase plus xanthine oxidase activity. 2,6-Dichlorophenol-indophenol has been used as an electron acceptor in this aasay. However, it was found in this study that it acts as an effective competitive inhibitor for xanthine oxidase. We conclude that methylene blue is the electron acceptor of choice in the fluorometric assays for xanthine oxidase.
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