2649. Measles-Containing Vaccination Resulted in a Balanced Cytokine Profile Without Evidence of Immunosuppression in Healthy 12-Month-Old Children

Abstract

BackgroundMeasles virus infection results in immune activation, viral clearance and lifelong immunity. In addition, there is an immunosuppressive state defined by type 2 skewing of CD4+ T‐cell cytokine production and induction of regulatory T cells with reduced dendritic cell (DC) activation in the recovery phase. Studies following measles immunization show conflicting immune profiles. To more robustly interrogate and define specific functional cytokine profiles, this study evaluated cytokine profiles in 12-month old infants before and after primary MMR vaccination.MethodsCytokine profiles using luminex assay (62-plex; eBioscience) were measured in 65 infants before and 42 days after MMR vaccination administered at 12 months of age as part of a randomized clinical trial. Mean cytokine percentages of children with increased or decreased concentrations of each cytokine in the post sample compared with the levels in the pre sample were evaluated using Student’s t-test. Cytokines were arranged into dominant CD4+ T-cell type, Th1, Th2, and T regulatory (Treg) and those produced by DC.ResultsNo dominant cytokine pattern emerged following measles immunization, with a balanced profile. The mean percentage of children with increased and decreased concentrations (pg/mL) of signature CD 4+ T-cell Th1 (tumor necrosis factor alpha [TNFa], interferon gamma [IFNg]), Th2 (Interleukin [IL] IL5, IL4, IL13), Treg (IL10, transforming growth factor-β TCFb) and DC (IL12P40 and IL12P70) cytokines were equivalent when measured at 42 days after MMR vaccine compared with levels before vaccine (Table 1) (P ≥ 0.05 for all comparisons).ConclusionIn contrast to data demonstrating an immune suppression profile following measles disease, measles-containing vaccine did not suppress Th1 CD4+ T-cell and DC cytokines or promote Th2 and Treg CD4+ T-cell cytokines measured 42 days after vaccination. The cytokine profile represents one of balance and homeostasis. This study supports the data that show measles vaccine does not cause immunosuppression in healthy infants. Disclosures All authors: No reported disclosures.