260-POS

Abstract

Objectives To know the difference of eNOS expression in the exposure of normal serum with preeclamptic serum and Syzygium cumini extract. Methods This study used HUVEC cultures that were already exposed to preeclamptic serum as HUVEC PE model that has dysfunction. Endothelial cells were taken from umbilical cord of female newborns and culture made until a confluent culture was formed. HUVEC cultures were divided into six groups: (1) HUVECs+ Normal Serum (Control), (2) HUVECs+ Preeclamptic serum (HUVECs PE model), (3) HUVECs PE Model+ S.cumini extract 50 ppm, (4) HUVECs PE Model+ S. cumini extract 100 ppm, (5) HUVECs PE Model+ S. cumini extract 200 ppm, (6) HUVECs PE Model+ S. cumini extract 400 ppm. The cultures were incubated for 24 h; immunocytochemistry-painted using primary antibody was used. Endothelial NOS expression was observed using microscopy. Results When eNOS expression in group 1 was compared to group 2 and 5, there were significant differences; on the other hand there were no significant differences between group 1 and group 3, 4, and 6. Group 2 was significantly different from group 5 and 6, not significantly different from group 3 and 4. Group 3 was significantly different from group 5 and 6 but not significantly different from group 4. In group 4, 5 and 6 there were significant differences among each other. In the observation of HUVEC culture that was being exposed by S. cumini it was found that higher S. cumini dosage is given, the higher eNOS expression. Conclusions There were significant differences of eNOS expression between HUVECs preeclamptic model exposed to S. cumini. In HUVECs preeclamptic model exposed by S. cumini, the higher S. cumini dosage the higher eNOS expression. Disclosures K. Keman: None. S. Candra WB: None. T. Olfah: None. T. Olfah: None.