26] Vectors for constitutive and inducible gene expression in yeast

Abstract

Publisher Summary This chapter describes vector systems for constitutive and inducible gene expression in Saccharomyces cerevisiae . Three new plasmids (pG-1, pG-2, and pG-3) are constructed that direct high-level constitutive gene expression in yeast. These vectors, derived from plasmids originally developed for expression of the rat glucocorticoid receptor cDNA in yeast, contain the very efficient yeast glyceraldehyde-3-phosphate dehydrogenase promoter. Each plasmid contains the yeast TRPI gene and 2-μm origin of replication and the ampicillin resistance gene and prokaryotic origin of replication from pUC18. In addition, a hormone-inducible expression vector is discussed in the chapter whose low basal promoter activity is strongly enhanced by the addition of glucocorticoids to yeast cells expressing the glucocorticoid receptor.