Abstract
Publisher Summary Using conductance measurements, the number of microorganisms left on substrata after enzyme treatment can be evaluated without removal of the cells from the substrata, simply by immersing the substrata in a conductance measuring cell. This chapter describes a method based on conductance measurements for evaluating the enzymatic degradation of microbial biofilm. The chapter describes Malthus method that is useful for the evaluation of enzyme activity against microbial biofilm as the number of microorganisms left on the substratum after enzyme treatment can be evaluated and enumerated without removal of the cells from the substratum. Using this method, bactericidal activity can be evaluated against biofilm cells without removal of the cells from the substratum by simply placing the substratum with biofilm in an conductance measuring cell and immersing the electrodes. The described method was particularly useful for the evaluation of enzymatic degradation of biofilm, as cells left on the substratum after enzyme treatment was more detachable than untreated biofilm cells.
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