Abstract
This chapter discusses the spectrophotometric assay of oxygen consumption. The spectrophotometric assay of oxygen consumption (uptake) is based on the utilization of oxyhemoglobin to serve both as a source of oxygen and as an indicator of respiration. Deoxygenation of HbO 2 is accompanied by changes in optical density at different wavelengths. The advantages of the spectrophotometric method consist in its wide range of sensitivity as well as in its relative simplicity. By decreasing the reaction volume down to I nl it has been possible to measure the oxygen uptake of one single cell. Any spectrophotometer or photometer supplied with a mercury lamp and corresponding interference filter can prove suitable for oxygen-consumption measurements. The use of commercial oxyhemoglobin analyzers might be extended to the oxygen-consumption assays. The expected applications of the spectrophotometric method may be divided into two groups, depending on the degree of technical complexity involved: assays in the usual range of volumes (0.2–1.0 ml), using general purpose instruments and special assays, requiring equipment of higher technical standard.
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