26] Separation of cytoplasmic and mitochondrial elongation factors from yeast

Abstract

Publisher Summary This chapter describes both methods of isolation of mitochondrial and cytoplasmic elongation factors from yeast cells. A brief method for the isolation of mitochondrial ribosomes is also included. The chapter puts more emphasis on the separation of the two sets of elongation factors as well as on the isolation and purification of the mitochondrial elongation factors. Because of parallels between bacterial and mitochondrial elongation factors, the nomenclature for prokaryotic elongation factors is used for the latter: EF T is the aminoacyl-tRNA binding factor, and EF G is the peptidyl translocase. For the cytoplasmic elongation factors, EF 1 and EF 2 are used, as generally applied in eukaryotic protein synthesis.