Abstract
This research was aimed to study histopathology of rat kidney infected with Trypanosoma evansi after administration of sernai leaves extract (Wedelia bifolora). The samples used were kidneys of 25 male rats strain Wistar which were equally divided into 5 treatment groups. Group P1 was uninfected and untreated control, group P2 was infected with 1x103 T. evansi but not sernai leaves extract, rats from groups P3, P4, and P5 were infected with 1x103 T. evansi and given sernai leaves extract with the dose of 30 mg/kg bw, 45 mg/kg bw, and 60 mg/kg bw respectively. The sernai extract was administered orally using a stomach tube for 3 consecutive days. The next day after last treatment, all rats were sacrificed and necropsied. Kidneys were collected for histopathological examination such as degeneration and necrosis of tubules cells, convoluted contortus duct lumen, and adhesion and atrophy of glomerulus. Sernai leaves extract with the dose of 45 mg/kg bw was the most effective dose to prevent kidney damage due to T. evansi infection.
Highlights
study histopathology of rat kidney infected with Trypanosoma evansi after administration
kidneys of 25 male rats strain Wistar which were equally divided into 5 treatment groups
group P2 was infected with 1x103 T. evansi
Summary
Penelitian ini menggunakan ginjal yang diambil dari 25 ekor tikus putih (Rattus novergicus) jantan dengan berat badan 80-100 g berumur tiga bulan yang telah diinfeksi T. evansi isolat Madura dosis 1x103 dan pemberian ekstrak daun sernai. Kelompok pertama adalah kelompok kontrol negatif (P1) tanpa diinfeksi T. evansi dan tanpa pemberian ekstrak daun sernai. Pada hari ke-4, semua tikus dieutanasia dengan menggunakan kloroform dan diambil ginjalnya untuk dilakuan perendaman di dalam pengawet. Sampel daun sernai yang digunakan merupakan tumbuhan yang berumur lebih dari satu tahun dan panjang tanaman telah lebih dari 0,5 meter. Pembuatan ekstrak sernai diawali dengan proses maserasi 3x24 jam terhadap 5 kg daun sernai yang sudah dikeringkan dan dicincang halus, lalu dilarutkan dengan metanol dengan perbandingan 11:100 gram daun. Ektrak sernai yang telah diperoleh diberikan pada tikus sesuai dengan perlakuan dengan menggunakan sonde lambung
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