256 EVALUATION OF INCREASED VOIDING FREQUENCY IN MICE WITH TYPE 2 DIABETES MELLITUS

Abstract

creased at 1 week and remained doubled at 4 weeks during continuous CMG. This was paralleled by similar increases in bladder compliance. A striking reduction (50%) in EUS EMG activity during filling was also seen with PNC. Isovolumetric CMG in the PNC groups revealed a 40% reduction in peak bladder contraction pressures over baseline and a 30% reduction in isovolumetric bladder contraction areas under the curves. CONCLUSIONS: In this preliminary study, we have shown that bilateral pelvic nerve crush in the rat is a promising, long-lasting model of UAB, demonstrating both bladder and outlet dysfunction consistent with pelvic nerve denervation. Increased ICI, bladder compliance, and decreased EUS activity are consistent with decreased afferent sensitivity with PNC. Decreased bladder contraction strength is consistent with compromised efferent function. By establishing a chronic rat model for UAB in otherwise normal animals, we can test potential therapies for treating UAB and voiding dysfunction, thereby addressing both afferent and efferent dysfunction.