252 BIOCHEMICAL SERUM PROFILE AND LAPAROSCOPIC OVUM PICKUP CORRELATION IN ANGLO NUBIAN GOATS FED WITH DIFFERENT FAT SOURCES

Abstract

The aim of the present study was to evaluate the correlations between biochemical serum profile with total number of aspirated follicles (AF), oocytes recovered (OR), number of corpus lutea (CL), oocyte morphology quality, and oocyte in vitro maturation (IVM) rate. Eighteen goats were randomly divided into 3 groups based on treatment diets, with different fat sources [soy oil (SG, n = 6), linseed (LG, n = 6), and Megalac® (MG, n = 6)], formulated with 40% concentrate, 60% corn silage, of which 4% was ether extract from fat sources. The animals were submitted to a 15-day adaptation period and then a 70-day experimental period. The laparoscopic ovum pickup (LOPU) was performed after 36 h of an ovarian superstimulation protocol [FSH (80 mg) + eCG (300 IU)] on Day 42 (LOPU1) and Day 70 (LOPU2) of the trial. The number of AF, and the presence and number of CL were evaluated during the LOPU procedure. Blood samples were taken at 14 days, 7 days, and just before each LOPU, and grouped as P1 (mean of blood samples results obtained before LOPU1) and P2 (LOPU2); serum metabolites analysed were total cholesterol, triglycerides, aspartate aminotransferase (AST), creatine kinase, and plasma glucose. The recovered oocytes were classified by morphological appearance, and only G1 (homogenous ooplasma and at least 3 layers of cumulus cells), G2 (homogenous ooplasma and at least 1 complete layers of cumulus cells), and G3 (homogenous ooplasma and incomplete layers or no cumulus cells) were submitted to IVM. The oocytes were incubated in maturation media (TCM 199 supplemented with 10% heat inactivated goat serum) at 38.5°C and 5% CO2 for 27 h. Then, oocytes were subsequently fixed and stained on Hoechst 33342 and analysed using a fluorescent microscope for nuclear maturation. The IVM and analysis were performed for each individual. Pearson correlation test (P < 0.05) was applied in R® software between serum metabolites and LOPU variables. The triglycerides, creatine kinase, and glucose were not correlated with any LOPU variables. The total cholesterol was negatively correlated with G1, G2, and CL, and positively correlated with OR and G3 with determination coefficients (R) of –0.28, –0.38, –0.24, 0.25, and 0.44, respectively. The AST were negatively correlated with AF, G1, G2, and IVM, with R of –0.11, –0.43, –0.13, and –0.16, respectively. In conclusion, these results are suggestive that high serum levels of cholesterol and AST may interfere with oocyte quality, indicating a negative hyperlipidemia effect on reproductive efficiency as reported in cows.Financial support was provided by CNPq and FAPESP.