Abstract
Surface enhanced laser desorption/ionisation-time of flight (SELDI-TOF) mass spectrometry (MS) is an array based proteomic technology. It combines a variety of chromatographic surfaces that bind proteins from biological mixtures according to physicochemical properties with MS analysis. The relative expression of proteins at specific molecular weights can then be compared among different samples. The aim of this work is to establish protein profiles in Cystic Fibrosis (CF) serum and airway cell lysate for biomarker identification. These proteins may serve as diagnostic/prognostic markers or even as new targets for CF therapy. Serum and nasal epithelial cells (collected by brushing) were obtained from CF patients (n 13/15) and controls (n 11/9). Protein extracts were applied to a range ProteinChip surfaces (anionic exchange, cationic exchange and metal affinity) and analysed by SELDI-TOF-MS. The mass spectral data demonstrate a larger group of proteins with differential expression in serum compared to nasal cells lysate. 45 peaks (p < 0.05) differentiated CF serum from control. 9 peaks (p < 0.05) differentiated CF nasal cell lysate from control. Further work is required to validate these results in a larger cohort of CF patients and to identify candidate biomarkers with MS protein characterisation. Work supported by FCT/FEDER research grants (POCTI/SAU-MMO/56163/2004). PA is a recipient of FCT doctoral fellowship. CB and DP collaborated equally to supervision of the experiments. 2. CFTR Cell biology~Physiology
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