Abstract
The association of the receptor–estrogen (RE) complex with nuclear binding sites is considered to be of fundamental importance in the mechanism of action of estrogen. This chapter has developed a method for the determination of the concentration of RE in the nuclear fraction of estrogen target tissues, which relies on the exchange of [ 3 H] estradiol with estradiol that is bound to the nuclear fraction. This method permits an evaluation of the concentration of nuclear RE under in vivo conditions and as a function of non-labeled estrogenic compounds. The [ 3 H] estradiol exchange assay can be used to determine the number of specific nuclear binding sites in estrogen-sensitive tissues and permits to examine the influence of endogenous and exogenous estrogen on the concentration of nuclear RE and the concentration-time-response parameters of estrogen action.
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