242 ACINETOBACTER BAUMANNII OUTER MEMBRANE PROTEIN A ENHANCE IMMUNE RESPONSES TO RENAL CELL CARCINOMA VACCINES TARGETING CARBONIC ANHYDRASE IX

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research1 Apr 2011242 ACINETOBACTER BAUMANNII OUTER MEMBRANE PROTEIN A ENHANCE IMMUNE RESPONSES TO RENAL CELL CARCINOMA VACCINES TARGETING CARBONIC ANHYDRASE IX Duk-Yoon Kim, Bora Kim, Soo-Hee Kim, Jechul Lee, Jun-chul Kim, Choal-Hee Park, and Eun-kyoung Yang Duk-Yoon KimDuk-Yoon Kim Daegu, Korea, Republic of More articles by this author , Bora KimBora Kim Daegu, Korea, Republic of More articles by this author , Soo-Hee KimSoo-Hee Kim Daegu, Korea, Republic of More articles by this author , Jechul LeeJechul Lee Daegu, Korea, Republic of More articles by this author , Jun-chul KimJun-chul Kim Buchon, Korea, Republic of More articles by this author , Choal-Hee ParkChoal-Hee Park Daegu, Korea, Republic of More articles by this author , and Eun-kyoung YangEun-kyoung Yang Daegu, Korea, Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.311AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES However several immunotherapeutic strategies targeting carbonic anhydrase IX (CA9) have been evaluated for treatment of renal cell carcinoma (RCC), the efficacy of dendritic cell (DC)-based vaccinations against RCC is less than expected. The aim of this study was to examine if bacterial outer membrane proteins (Omps) increase the antitumor effect of CA9 in mouse RCC model. METHODS The recombinant OmpA from Acinetobacter baumannii (AbOmpA) was used for immunoadjuvants. Male BALB/c mice of 7 week old were injected subcutaneously with 5 X 105 CA9-expressed RENCA cells and the vaccines were administered on day 3, 10, and 17 after tumor injection. We compared the effect of different conditioning regimens of the murine bone marrow-derived DCs pulsed with tumor lysate alone or in combination with AbOmpA. Tumor growth was monitored individually every 3–4 days by measuring three perpendicular dimensions of each tumor and calculating tumor volume. Cytokine productions were measured using ELISA. RESULTS Pulsing with CA9 and AbOmpA elicites production of Th1-promoting interleukin-12 from DCs and enhances the capacity of DCs to stimulate immune responses. Compared with DCs pulsed with RENCA tumor lysate, the DCs pulsed with CA9 and AbOmpA simultaneously produced more powerful immune reactions. In a therapeutic model of murine RCC, DCs pulsed with CA9 and AbOmpA at the same time significantly decreased tumor growth. CONCLUSIONS These results reveal that DCs pulsed with CA9 and AbOmpA simultaneously are more effective inducer of antitumor immune responses in mouse model and may provide a new strategy of DC-based vaccines for the treatment of RCC. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e98 Peer Review Report Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Duk-Yoon Kim Daegu, Korea, Republic of More articles by this author Bora Kim Daegu, Korea, Republic of More articles by this author Soo-Hee Kim Daegu, Korea, Republic of More articles by this author Jechul Lee Daegu, Korea, Republic of More articles by this author Jun-chul Kim Buchon, Korea, Republic of More articles by this author Choal-Hee Park Daegu, Korea, Republic of More articles by this author Eun-kyoung Yang Daegu, Korea, Republic of More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...