242 A Novel Co-culture System of Multiple Cell Types for Mimicking Skin Microenvironment

Abstract

Recent advances have been made toward developing three-dimensional (3D) in vitro human skin models, including skin characteristics in terms of epidermal and dermal structures, barrier functions, cell migration, and cell-to-cell interaction in the 3D microenvironment. However, current 3D in vitro human skin models have been a limitation that monitors long-term real-time for validating in vitro, at least, with the co-culture of two main types of skin cells. To overcome these limitations, we investigated whether decellularized bioscaffolds of non-human origin provide a naturally derived microenvironment for skin-associated cells to be adhesion, migration, growth, and differentiation their great merits.