Abstract
This chapter describes the use of lac activator proteins (LAPs) for regulated expression of oncogenes. Inducible expression systems have a variety of uses in the study of oncogenes. Inducible expression systems can be used to establish conditionally transformed cell lines by regulating expression of an oncogene. Such cell lines can be used to study various aspects of the transformation process including the temporal requirements for morphological transformation or reversion to the untransformed state. This chapter outlines the LAP system, the currently available vectors, and their use in developing conditionally transformed cell lines using LAP-inducible oncogenes. The LAP system is based on the isopropyl β-d-thiogalactoside (IPTG)- regulated lac repressor/LAP. Fusion of the lac repressor DNA-binding protein with a mammalian transcriptional activation domain results in the generation of an IPTG-regulated transcription factor. The LAP proteins have the ability to activate a number of mammalian promoters when the promoters are linked to lac operators.
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