Abstract
Publisher Summary This chapter describes the preparation of the biocatalysts and some of their industrial applications. Cellulose acetate derivatives, having a polymerization degree of around 300, can be used for biocatalyst immobilization. Enzymatic cells have been entrapped in cellulose triacetate (CTA) fibers following the procedure used for the immobilization of free enzymes, but the results obtained were not satisfactory. Cellulose diacetate (CDA) is found to be suitable for the entrapment of cells because of its physical and chemical properties. CDA has a degree of polymerization lower than CTA, it is soluble both in acetone and acetone-water mixtures, and coagulates in water taking different physical shapes (fibers, disks, and beads) according to the viscosity of the extruded dispersion. The entrapment is therefore carried out under mild conditions generally compatible with the enzymatic activity.
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