Abstract
Extracorporeal perfusion of canine kidneys for periods of 24 to 72 hours were consistently successful. The functional viability of the perfused kidneys was proved by reimplanting the perfused kidneys and simultaneously removing the contralateral kidneys. The essential features of successful perfusion are: (1) a pulsatile pump; (2) moderate hypothermia (8-12°C); (3) a buffered perfusate of canine plasma to which are added magnesium sulphate, dextrose, insulin, penicillin, and hydrocortisone; (4) microfiltration of the perfusate before use; (5) oxygenation by means of a membrane oxygenator to limit the air-fluid interphase; and (6) control of pH, temperature, PO 2, PCO 2, and flowrates during perfusion. Since a 24-hour to 72-hour period is adequate for preparing a recipient and such preliminaries as tissue-typing, the details of the technique in twelve consecutive perfusions, six for 24 hours and six for 72 hours are reported.
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