Abstract
DNA was extracted from leaves of various Malus genotypes and used to screen synthetic decamer oligonucleotide primers. Samples from the following two groups were bulked: 1) seven scab-susceptible apple cultivars, and 2) 15 scab-resistant apple genotypes derived by introgressive hybridization from the previous group of cultivars. A third sample consisted of DNA extracted from Malus floribunda Sieb. clone 821, the original source of apple scab resistance for all genotypes in the second group. A total of 59 primers from kits A, L, and R (Operon Technologies) were screened. Amplified fragments were obtained for 93% of the primers tested, while random amplified polymorphic DNA (RAPD) fragments were detected among samples for 76% of the primers. One primer, A15, amplified a unique band in both M. floribunda clone 821 and the bulked scab-resistant sample. This RAPD marker, designated OA15900, identifies an amplified, introgressed fragment that likely corresponds to a region of the genome that may serve as a modifier for the scab resistance gene block V, derived from M. floribunda clone 821.
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