2321 COMPARATIVE ANALYSIS OF PROSTATE CANCER ANTIGEN 3 MRNA EXPRESSION IN BENIGN PERIPHERAL PROSTATIC TISSUE, CANCER AND ISOLATED OR CANCER-ASSOCIATED HIGH GRADE PROSTATIC INTRAEPITHELIAL NEOPLASIA

Abstract

You have accessJournal of UrologyProstate Cancer: Markers1 Apr 20112321 COMPARATIVE ANALYSIS OF PROSTATE CANCER ANTIGEN 3 MRNA EXPRESSION IN BENIGN PERIPHERAL PROSTATIC TISSUE, CANCER AND ISOLATED OR CANCER-ASSOCIATED HIGH GRADE PROSTATIC INTRAEPITHELIAL NEOPLASIA M. Teresa Quiles, Maria Antònia Arbós, Inés M. de Torres, Carmen Blázquez, Juan M. Bastarós, José Placer, Andreas Doll, Jaume Reventós, and Juan Morote M. Teresa QuilesM. Teresa Quiles Barcelona, Spain More articles by this author , Maria Antònia ArbósMaria Antònia Arbós Barcelona, Spain More articles by this author , Inés M. de TorresInés M. de Torres Barcelona, Spain More articles by this author , Carmen BlázquezCarmen Blázquez Barcelona, Spain More articles by this author , Juan M. BastarósJuan M. Bastarós Barcelona, Spain More articles by this author , José PlacerJosé Placer Barcelona, Spain More articles by this author , Andreas DollAndreas Doll Barcelona, Spain More articles by this author , Jaume ReventósJaume Reventós Barcelona, Spain More articles by this author , and Juan MoroteJuan Morote Barcelona, Spain More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.2568AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Prostate cancer antigen 3 (PCA3) score in urine after prostate-massage is a novel tool to increase prostate specific antigen (PSA) specificity especially in men with repeat prostate biopsy indication. Current data suggest a lower efficacy of PCA3 score in men with HGPIN in the first biopsy1. The objective of this study was to analyze the PCA3 gene expression in benign tissue (BT), cancer (CA) and HGPIN lesions (isolated: IHGPIN and cancer-associated: AHGPIN). METHODS Total mRNA was isolated and amplified from laser-assisted microdissected foci of BT, CA and AHGPIN located in 15 radical prostatectomy frozen specimens and IHGPIN located in 13 radical cistoprostatectomy paraffin-embedded blocks from patients with bladder cancer. PCA3 mRNA amounts were measured by real-time qPCR (Sybr Green I chemistry). PCA3 mRNA amounts were normalized to two reference genes: small nucleolar RNA (SNORD) and Rho GTPase activating protein (ARHGAP). Target gene levels were determined using efficiency-corrected calibrator normalized relative quantification. Comparison among tissues was performed using non-parametric Friedman test followed by post-hoc Dunn's multiple comparison test. RESULTS PCA3 mRNA expression was detected in 80% of BT samples, 86.7% of AHGPIN and 92.9% of CA tissues. PCA3 mean quantitative expression was 11.4 (p=0.009) and 20.7-fold increased (p=0.0002) in AHGPIN and CA related to BT samples. Qualitative PCA3 mRNA expression was detected in 61.5% of IHGPIN samples. CONCLUSIONS We have demonstrated that PCA3 is not a specific gene of prostate cancer. PCA3 mRNA expression is significantly increased in cancer-associated HGPIN lesions as well as qualitatively expressed in isolated HGPIN lesions. After these findings and the previous clinical observation of a lower efficacy of PCA3 score in men with previous HGPIN1 we suggest that specific threshold of PCA3 score should be established in those men. References 1 : Behavior of the PCA3 gene in the urine of men with high grade prostatic intraepithelial neoplasia. World J Urol2010 Jul 7; . [Epub ahead of print]. Google Scholar © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e930-e931 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information M. Teresa Quiles Barcelona, Spain More articles by this author Maria Antònia Arbós Barcelona, Spain More articles by this author Inés M. de Torres Barcelona, Spain More articles by this author Carmen Blázquez Barcelona, Spain More articles by this author Juan M. Bastarós Barcelona, Spain More articles by this author José Placer Barcelona, Spain More articles by this author Andreas Doll Barcelona, Spain More articles by this author Jaume Reventós Barcelona, Spain More articles by this author Juan Morote Barcelona, Spain More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...