232 Immunomodulation with AM3 and antioxidants creates an adequate framework for skin repair and decreases the monocyte proinflammatory stage in smoker women

Abstract

Tobacco smoke has been considered a crucial factor in promoting skin and systemic aging and is also associated with the development of a low-level, systemic, chronic inflammation known as “inflammaging” where monocytes play a pivotal role. In our study, 34 healthy smoker middle- aged women were 1:1 randomly assigned to AM3 plus antioxidants and placebo treatment for three months. Peripheral mononuclear blood cells count, and cutaneous biopsy were obtained before and after the treatment and flow cytometry and immunohystologycal studies, respectively, were performed. AM3 is a glucomannan of natural origin from Candida utilis cell wall. AM3 plus antioxidants treatment significantly reduced the monocyte production of the proinflammatory IL-1, TNFα and IL-6 cytokines and also increased the regulatory IL-10 in middle-aged smoker women. The active combination also stimulated a significative increase in reticular dermis depth as well as an increase in the expression of CD117 and CD31. CD117 (also known as c-kit) is a tyrosine kinase receptor that is found in mast cells and whose expression may correlate with the increase in reticular dermis depth due to the production of cytokines such as TGFβ. As seen in fibroblasts, TGFβ-1 and TGFβ-2 are potent enhancers of elastin gene expression. On the other hand, CD31 is a glycoprotein present on the surface of endothelial cells. The increase in the expression of CD31 is related with an enhancement in dermis vascularization. In conclusion, AM3 and antioxidants treatment reduces the systemic proinflammatory monocyte disturbance of heathy smoker middle- aged women and favors the generation of cutaneous repair mechanisms.