232 Csf Cytokine and Chemokine Profiles in Japanese Patients with West Syndrome

Abstract

Background and aims: We assessed the cytokine and chemokine profiles of cerebrospinal fluid (CSF) in patients with West syndrome to elucidate whether immunological processes are concerned with the pathophysiology of West syndrome.Methods: We analyzed the CSF levels of Japanese ten patients with West syndrome (WS), eight Influenza virus-associated encephalopathy (IE), as a representative disease with high cytokines storms and twelve controls (cont). All samples of CSF were obtained the first 24 hours after the tonic spasms with informed consent. Seventeen items were measured using the Bio-Plex Multiplex Cytokine Assay, Interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, G-CSF, GM-CSF, INF-γ, MCP-1, MIP-1β and TNF-α. Statistical differences between three independent groups were tested using the nonparametric Kruskal-Wallis test followed by paired testing using the Mann-Whitney test. A p value of 0.05 was considered significant.Results: All of the CSF of cytokine and chemokine levels in the WS and IE groups was significantly higher than those in the cont group. The CSF levels of IL-6, IL-8, G-CSF, INF-γ and TNF-α in the WS group were significantly lower than those in the IE group. No significant differences of the other cytokines were detected between the WS and IE groups.Conclusions: Our results demonstrate that the increased CSF levels of cytokine and chemokine profiles in patients with West syndrome. A larger study should be conducted to clarify which cytokines are mainly concerned with the pathophysiology of WS.