Abstract
This chapter discusses a transient expression system that is suitable for the study of the expression of the interstitial retinoid-binding protein (IRBP) gene. This system is applicable for gene expression studies of photoreceptor-specific proteins in retinoblastoma cells. A retinoblastoma cell line, WERI-RB1, is used to dissect the regulatory sequences that are responsible for the expression of human IRBP in vivo . IRBP is a large and elongated glycoprotein that is universally distributed throughout the vertebrates. It is synthesized by the photoreceptor cells in the retina and is secreted into the interphotoreceptor matrix. The major function of IRBP is to transport 11-cis- and all-trans-retinoids between the neural retina and the retinal pigment epithelium. The chapter discusses the optimization of WERI-RB1 transfections. The conditions required for optimal transfection vary depending on each individual cell line. The amount of the plasmid construct and the liposome used for transfection should be adjusted so that the transfected cells produce maximum chloramphenicol acetyltransferase (CAT) enzyme activity for a given promoter.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
