Abstract
This study aimed to determine the effect of temu kunci’s extract (Boesenbergia pandurata) on the growth of Pseudomonas aeruginosa in vitro. This research was conducted using seven treatments with three replications. The experiment was carried out using Kirby Bauer’s method, the number of bacteria used was in accordance with the standards of Mc Farland 1. Blank discs were soaked in temu kunci’s extract in concentration of 10, 20, 30, 40, and 50%. Streptomycin was used as a positive control and sterile aquades as a negative control. All the discs were placed on the surface of bacteria cultured on MHA and incubated for 24 hours at 37 °C. Diameter of inhibition zone formed was measured by using a caliper. Data were analyzed descriptively. The average diameter of inhibition zone of temu kunci’s extract with a concentration of 10, 20, 30, 40 and 50% were 8.00, 12.30, 13.85, 19.16, and 19.86 mm, respectively. The average diameter of inhibition zone formed on the positive control and negative control were 14,25 and 0 mm, respectively. The conclusion showed that the temu kunci’s extract (Boesenbergia pandurata) has the ability to inhibit the growth of Pseudomonas aeruginosa.
Highlights
Infeksi adalah proses berkembangnya agen infeksi ke dalam tubuh hewan dan manusia
This study aimed to determine the effect of temu kunci
The experiment was carried out using Kirby Bauer's method
Summary
Rimpang temu kunci dicuci bersih dengan air, dipotong kecil-kecil, dikeringkan dengan diangin-anginkan selama 7 hari. Selanjutnya dihaluskan dengan menggunakan blender hingga diperoleh berupa serbuk. Hasil blender dikeringanginkan kembali selama 3 hari, selanjutnya direndam menggunakan pelarut etanol 96% dalam wadah tertutup dan Florentina Magdalena Girsang, dkk dibiarkan selama 3 hari. Rimpang temu kunci disaring menggunakan corong dan kertas saring sehingga diperoleh berupa filtrat. Selanjutnya dilakukan evaporasi dengan menggunakan vacuum rotary evaporator. Hasil maserat dimasukkan ke dalam wadah dan ditutup rapat
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