Abstract
In the present work, electrochemical impedance immunosensor is developed for detection of myoglobin. The procedure involves the immobilization of myoglobin antibodies on the interdigitated gold electrodes (IDE) using alkanethiol self-assembled monolayer (SAM) for binding myoglobin antigens available in the blood serum/aqueous solution. Then the sensor system is characterised using alternating current (AC) electrochemical impedance spectroscopy (EIS), in which the change in impedance spectra is observed across the frequency range of 0.1 Hz to 10 MHz. This work mainly focuses on understanding and improving the impedance immunosensor for better sensitivity readings (~1 ng/mL) by adjusting the interdigitated electrode configuration, incubation times, reagent sample volumes and immobilisation protocols. Myoglobin is one of the premature indentifying cardiac protein markers to monitor minor heart attacks. Hence, the developed immunosensor has potential of using as a point-of-care diagnostic device. The protocol developed in this work can be useful for detecting other cardiac markers like Troponin and CK-MB by using respective antibodies.
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