2254 BLOCKING OF BLOOD GROUP A- OR B-ANTIGEN BY BLOOD GROUP SPECIFIC BINDING PEPTIDE: NEW STRATEGY BY OVERCOMING ABO INCOMPATIBLE KIDNEY TRANSPLANTATION

Abstract

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation, Vascular Surgery1 Apr 20112254 BLOCKING OF BLOOD GROUP A- OR B-ANTIGEN BY BLOOD GROUP SPECIFIC BINDING PEPTIDE: NEW STRATEGY BY OVERCOMING ABO INCOMPATIBLE KIDNEY TRANSPLANTATION Tohru Yoneyama, Shingo Hatakeyama, Kengo Imanishi, Noriko Tokui, Teppei Okamoto, Naoki Sugiyama, Yuichiro Suzuki, Shigemasa Kudo, Takahiro Yoneyama, Kazuyuki Mori, Takuya Koie, Noritaka Kamimura, and Chikara Ohyama Tohru YoneyamaTohru Yoneyama Hirosaki, Japan More articles by this author , Shingo HatakeyamaShingo Hatakeyama Hirosaki, Japan More articles by this author , Kengo ImanishiKengo Imanishi Hirosaki, Japan More articles by this author , Noriko TokuiNoriko Tokui Hirosaki, Japan More articles by this author , Teppei OkamotoTeppei Okamoto Hirosaki, Japan More articles by this author , Naoki SugiyamaNaoki Sugiyama Hirosaki, Japan More articles by this author , Yuichiro SuzukiYuichiro Suzuki Hirosaki, Japan More articles by this author , Shigemasa KudoShigemasa Kudo Hirosaki, Japan More articles by this author , Takahiro YoneyamaTakahiro Yoneyama Hirosaki, Japan More articles by this author , Kazuyuki MoriKazuyuki Mori Hirosaki, Japan More articles by this author , Takuya KoieTakuya Koie Hirosaki, Japan More articles by this author , Noritaka KamimuraNoritaka Kamimura Hirosaki, Japan More articles by this author , and Chikara OhyamaChikara Ohyama Hirosaki, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.2496AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Antibody mediate rejection (AMR) is a major barrier in ABO-incompatible kidney transplantation (ABO-I KTx). AMR is triggered by anti-donor blood group A/B antibodies (Abs), which might bind to blood group A/B-antigen on the endothelium of the graft. The advent of new immunosuppressive agents and advances in immunosuppressive therapy have markedly improved outcomes ABO-I KTx. It is well known that various strategies have been performed to remove or to reduce anti-blood group Abs. However, blood group antigen-blocking treatments have not been tried in clinical application. In this study, we tried to indentify a peptide sequence which specifically binds to blood group antigen. METHODS We screened a blood group A- or B-antigen binding 7-mer peptide by T7 phage random peptide library display screening system. Next, we investigated whether A or B-antigen binding peptide can inhibit hemagglutination (HA) of blood group A/B red blood cells (RBCs) by anti-A or -B antibody. Furthermore, we investigated whether A/B-antigen binding peptide can bind to blood group A- or B-antigen on the paraffin-embedded and frozen kidney tissue section. RESULTS We identified eight 7-mer peptides which binds to blood group A (ASNKRPR, SRAPKPR, ARARKTG and RPRNPNK), blood group B (GKTRSK, ALRTRSK, SRSSTRK) or both blood group antigen (RMSRKLP) respectively. Blood group A/B binding peptides coated RBCs were inhibited HA activity by anti-A or B antibody. Furthermore, these peptides also bind to A/B-antigen on the microvascular, peritubular and glomerular endotherium of kidney. Binding pattern of peptide on kidney tissue was very similar to that of anti-blood group antibody. CONCLUSIONS These data indicating that blood group A/B-antigen on RBCs and on kidney tissues may neutralize by A- or B-antigen binding peptide. The neutralization of blood group antigen by blood group antigen binding peptide and might represent one strategy to overcome ABO-I KTx. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e904 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tohru Yoneyama Hirosaki, Japan More articles by this author Shingo Hatakeyama Hirosaki, Japan More articles by this author Kengo Imanishi Hirosaki, Japan More articles by this author Noriko Tokui Hirosaki, Japan More articles by this author Teppei Okamoto Hirosaki, Japan More articles by this author Naoki Sugiyama Hirosaki, Japan More articles by this author Yuichiro Suzuki Hirosaki, Japan More articles by this author Shigemasa Kudo Hirosaki, Japan More articles by this author Takahiro Yoneyama Hirosaki, Japan More articles by this author Kazuyuki Mori Hirosaki, Japan More articles by this author Takuya Koie Hirosaki, Japan More articles by this author Noritaka Kamimura Hirosaki, Japan More articles by this author Chikara Ohyama Hirosaki, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...