Abstract
Ascorbic acid is a small-molecular reductant with multiple functions in vivo and related to disease states. In previous work, we developed an ascorbic acid detection fluorescent probe, which employs covalent coupling of nitroxide with a fluorophore, leading to intramolecular quenching of fluorescence emission. However, this fluorescent probe showed short emission wavelength where is overlapped with the other in vivo fluorescent substances. To overcome this undesirable property, we focused on Nile-Blue used as a fluorophore and developed a long wavelength fluorescent probe for detecting ascorbic acid. First, we synthesized a long wavelength fluorescent probe “Nile-DiPy” for detection of ascorbic acid. To confirm the value of this probe, we measured the concentration of plasma ascorbic acid of Streptozotocin-induced diabetic mouse using Nile-DiPy. There was good correlation between the plasma ascorbic acid levels measured by the method using Nile-DiPy and by HPLC. These result indicate that Nile-DiPy could sensitively and conveniently detect ascorbic acid and be a useful bio-probe for understanding the role of ascorbic acid in disease states.
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