Abstract
A novel electrochemical technique for the detection of DNA sequence has been developed by a simple electrochemical deposition of chloro-2,2':6',2''-terpyridine platinum (II) chloride dihydrate (Ptcomplex) on a glassy carbon electrode. The intercalation of a Pt-complex to a double-stranded DNA (ds-DNA), which is formed with the complementary recognition of probe DNA to target DNA, caused to suppress the electroreduction of the Pt-complex due to the steric hindrance and decrease of the diffusion coefficient. Thus, the catalytic current for reducing proton by the platinum deposited on the glassy carbon electrode decreased with the increase of the concentration of target DNA. An amplified response for measuring the current for reducing proton as compared with that for reducing the Ptcomplex itself could provide the highly sensitive measurement of ds-DNA.
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