221 Functional Modulation of the Blood Brain Barrier

Abstract

Abstract INTRODUCTION Delivery of therapeutic agents to the brain is constrained by the blood-brain barrier (BBB). Previous work (Yarnitsky) suggested BBB permeability was increased with stimulation of the sphenopalatine ganglion (SPG). However, their model looked at FITC-dextran signal in CSF superfusate, a reflection of epithelial tight junctions at the blood-CSF barrier, and quantified BBB permeability using Evans blue, a marker insufficient for this role (Saunders). METHODS Experiments were conducted in Sprague-Dawley rats using 70 kDa FITC-dextran as a marker to quantify BBB permeability. Once anesthetized, the right femoral vein was exposed and catheterized. Next, SPG fibers were exposed behind the right eye and an electrode was hooked around those fibers. Stimulation occurred in blocks of 90 seconds of “on” time at 5 volts and 10 Hz followed by 60 seconds of “off” time. Injection of 0.1 mL of a 100 mg/mL concentration of FITC coincided with each “on” stimulation cycle; a total of 1.0 mL was injected per animal. Control animals were not stimulated, but had the same injection protocol as test animals. 90 seconds after the final injection, a blood sample was collected and the cerebrovasculature was flushed. Each brain was removed, equally divided, and homogenized. Post-centrifugation supernatant from blood and tissue homogenates was analyzed using fluorescent spectrometry. FITC concentrations were calculated using known standards. An uptake ratio was calculated by dividing sample FITC concentration by blood FITC concentration. RESULTS >Data from 4 control animals and 4 test animals demonstrated a significantly greater uptake ratio in test brains compared with control brains (P = 0.001). A significantly increased uptake ratio was also observed in both right and left hemispheres of test animals compared with controls (right, P = 0.03; left, P = 0.01). CONCLUSION Stimulation of SPG fibers significantly increased BBB permeability in both cerebral hemispheres of test animals when compared with controls.