220 Inhibition of lipid synthesis on human Caucasian, Asian and African sebocytes by honey from Aland island

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Sebum and lipids secretions play an important function in skin providing a lipidic layer to prevent water loss and contribute to skin barrier integrity by maintaining stratum corneum hydration. However, environmental aggression, psychological stress, climate change, nutrition diet, hormonal variation, bacterial infection trigger lipids over-production mediated by skin inflammation. The excess of sebum secretion leads to moderate skin disorders such as oily and shiny skin but also on severe disorders such as acne. Honey is one of the oldest natural products used by human and their activities on the skin proved by numerous studies and it is used in medical treatments but also as cosmetic ingredients. Honey contains more than 180 ingredients and are specific to bees and environment of hives making each honey particular. The molecular complexity of honey allows to offer a broad range of biological activities such as anti-oxidation, anti-inflammatory, anti-microbial. Based on these data and previous results, we studied the modulation of lipids synthesis exerted by honey from Aland island on human sebocytes from different ethnic background derived from human induced pluripotent stem cells. Cells were treated with arachidonic acid (AA) for triggering inflammation/lipid synthesis and with a concentration range of honey, then fixed, permeabilized and incubated with Bodipy and Hoechst fluorescent probes. After observation by epifluorescence microscope, a quantification of lipids was performed. AA treatment induced a strong and significant increase in lipid content and Aland honey significantly inhibited, in a dose-dependent manner, lipid synthesis on the three lines of human sebocytes. For the first time with human sebocytes from different ethnic background, we showed that Aland honey can strongly inhibit lipid production. This activity seems at least associated with inhibition of genes regulating sebogenesis such as PPARγ, DGAT2 and AWAT1. Our results make Aland honey a very good candidate for skin care dedicated to oily skin.