Abstract
This study aimed to determine the effect of garlic extract (Allium sativum) to the growth of Aeromonas hydrophila bacteria. Sample of A. hydrophila was obtained from the Fish Quarantine Station of the Aceh Fisheries Product Quality and Safety Control.Extraction of garlic was conducted by maceration method using ethanol solvent. This study used 6 treatments and consisted of three replications. Treatment I concentration of garlic extract 45%, treatment II with concentration of garlic extract 50%, treatment III concentration 55%, treatment IV concentration 60%. Positive control used chloramphenicol antibiotics and negative control used 96% ethanol. The test results showed that 60% garlic extract (A. sativum) was more effective in inhibiting the growth of A. hydrophila bacteria compared to other concentrations. The lowest effect was owned by 45%. Ethanol extract of garlic (A. sativum)had the ability to inhibit the growth of A. hydrophila because it has antibacterial compounds.
Highlights
Indikator keberhasilan dalam usaha budidaya ikan adalah kondisi kesehatan ikan
This study aimed to determine the effect of garlic extract
Sample of A. hydrophila was obtained from the Fish Quarantine Station of the Aceh Fisheries Product Quality
Summary
Metode yang digunakan pada penelitian ini untuk mengekstrak bawang putih (Allium sativum) adalah metode maserasi. Pada metode maserasi ini digunakan pelarut etanol 96%. Sebanyak 500 Gram bawang putih terlebih dahulu dikupas kulitnya dan dicuci bersih, dikeringanginkan, kemudian dihaluskan hingga menjadi serbuk kering. Serbuk kering direndam dalam 2 liter pelarut etanol 96% selama 3x24 jam. Pengadukan pada metode maserasi dilakukan sebanyak 12 kali selama 15 menit. Kemudian dilakukan penyaringan untuk memisahkan filtrat dari ampas. Hasil saringan kemudian diuapkan dengan vacuum rotary evaporator sampai kental. Ekstrak bawang putih tersebut disimpan dalam lemari es pada suhu 40C dan tidak terkena cahaya matahari langsung (Karina, 2013)
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