216: A maternal high fat diet reprograms the histone code to modulate fetal thyroid gene expression and thyroid associated proteins in a primate model of obesity

Abstract

to modulate fetal thyroid gene expression and thyroid associated proteins in a primate model of obesity Melissa Suter, Aishe Chen, Min Hu, Lori Showalter, Cynthia Shope, Kathleen Brown, Kevin Grove, Robert Lane, Kjersti Aagaard Baylor College of Medicine, ObstetricsG pregnant rats with saline drinking water and weekly injections of desoxycorticosterone acetate (PDS; preE rat model, n 9); and NP rats injected with MBG (7.65 microgram/kg/d, NPM, n 8). Plasma and urinary levels of Aldo were assayed in all groups using ELISA. (2) The effect of MBG on Aldo secretion was evaluated using cultured adrenal cells treated with DMSO (vehicle), 0.1, 1, 10 or 100 nM MBG. Aldo levels in culture media and cell lysates of MBG-treated adrenal cells were measured. (3) We recruited 17 PreE and 23 pregnant patients from the Scott & White Memorial Hospital. Aldo and MBG levels were assayed in patients plasma. RESULTS: (1) Rat plasma and urinary levels of Aldo were significantly lower in PDS and NPM compared to NP (NP: 578.5 / 152.4, PDS: 379.5 / 89.6, NPM: 348.7 / 91.3 g/mL for plasma; NP: 86.7 / 10.7, PDS: 52.0 / 5.2, NPM: 58.9 / 6 for urine, p 0.05 for each comparison). (2) Concentrations of MBG 1 nM significantly decreased Aldo secretion in cultured adrenal cells. (3) Mean plasma Aldo (398.6 / 115.8 g/mL) in PreE patients was significantly suppressed compared to NP women (548.4 / 137.5 g/mL), while, plasma MBG was significantly higher in preE compared to NP (preE: 59 / 17 & NP: 12 / 2 pg/mL) subjects. CONCLUSION: These data confirm that Aldo is reduced in preE model and women with preE. The inverse relationship of MBG and Aldo in the preE rat model and in adrenal cells in vitro and also in women with preE suggests that MBG may play a key role in the suppression of Aldo in PreE. Poster Session I Clinical Obstetrics, Medical-Surgical-Disease, Neonatology, Physiology-Endocrinology www.AJOG.org