Abstract

Single-cell RNA seq analyses on human epidermis have given new insights into cell fates of the tissue by characterizing the precise transcriptomic profile at single-cell scale, but information of spatial localizations of transcripts in each single cells is lacking. 3D transcriptomic analyses are therefore of great importance to characterize cell fates of the epidermis with spatial resolution. To study human epidermal biology and diseases, organotypic models can be grown using keratinocytes, which reproduce the architecture of the epidermis.

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