213 LOW EXPRESSION OF MICROPHTHALMIA-ASSOCIATED TRANSCRIPTION FACTOR, POTENTIAL MOLECULAR TARGET FOR INTERFERON-ALPHA SUSCEPTIBILITY, IS PREDICTOR FOR METASTASIS IN RENAL CELL CARCINOMA

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research II1 Apr 2010213 LOW EXPRESSION OF MICROPHTHALMIA-ASSOCIATED TRANSCRIPTION FACTOR, POTENTIAL MOLECULAR TARGET FOR INTERFERON-ALPHA SUSCEPTIBILITY, IS PREDICTOR FOR METASTASIS IN RENAL CELL CARCINOMA Toru Shimazui, Takahiro Kojima, Kazuhiro Yoshikawa, Yoshihiro Ami, Kazuhiko Uchida, and Hideyuki Akaza Toru ShimazuiToru Shimazui Tsukuba, Japan More articles by this author , Takahiro KojimaTakahiro Kojima Tsukuba, Japan More articles by this author , Kazuhiro YoshikawaKazuhiro Yoshikawa Nagakute, Japan More articles by this author , Yoshihiro AmiYoshihiro Ami Inashiki, Japan More articles by this author , Kazuhiko UchidaKazuhiko Uchida Tsukuba, Japan More articles by this author , and Hideyuki AkazaHideyuki Akaza Tsukuba, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.270AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We have previously reported that several candidate genes were selected in association with interferon-α (IFN-α) response in renal cell carcinoma (RCC) cell lines using microarray expression profiling (Cancer Sci 2007;98:529). Because expression profile of microphthalmia-associated transcription factor (MITF) correlated well with IFN-α-response status of RCC cell lines, we focused clinical significance of MITF expression in clinical specimens. In this study, we investigate correlation between expression level of MITF and presence and future occurrence of metastasis in patient with RCC. METHODS From 14 RCC cell lines and 65 RCC samples, RNA was extracted and used in this study. After transfection of cDNA of the MITF, alteration of gene expression level and IFN-α-susceptibility of transfectant were analyzed by quantitative PCR and WST-8 assay, respectively. In RCC samples, mRNA of MITF was measured and correlation between MITF expression level and clinico-pathological parameters were statistically analyzed. In patient without metastasis (M0 RCC) at the time of nephrectomy, correlation between MITF expression and future metastasis was analyzed. RESULTS MITF cDNA transfection into IFN-α-resistant RCC cell line resulted in elevation of MITF expression and acquisition of IFN-α-sensitivity. Statistical analysis revealed that low MITF mRNA expression is significantly correlated with presence of metastasis and poor survival of the patient (p<0.0258 and p=0.0065 (Figure), respectively). In addition, low MITF expression seemed to correlate with poor recurrence free survival of patients with M0 RCC. CONCLUSIONS MITF gene transfection provided elevation of IFN-α-sensitivity in RCC cell line, suggesting that this gene is a target molecule for modulation of IFN-α-response. Quantification of MITF mRNA expression might be clinically useful to predict metastasis and survival of patients with RCC. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e84 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Toru Shimazui Tsukuba, Japan More articles by this author Takahiro Kojima Tsukuba, Japan More articles by this author Kazuhiro Yoshikawa Nagakute, Japan More articles by this author Yoshihiro Ami Inashiki, Japan More articles by this author Kazuhiko Uchida Tsukuba, Japan More articles by this author Hideyuki Akaza Tsukuba, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...