2110-P: Increased Proinflammatory Eosinophils in Adipose Tissue of Metabolic Syndrome

Abstract

Metabolic Syndrome (MetS) is a common disorder that predisposes to type 2 diabetes mellitus (T2DM) and cardiovascular disease (ASCVD). Adipose tissue (AT) contributes significantly to increased inflammation and insulin resistance (IR) in MetS. Many studies illustrate the role of macrophages and lymphocytes in human SAT, but there is sparse data on other immune cells, especially eosinophils. Hence, in this report we investigated the abundance of eosinophils (EOS) in subcutaneous AT (SAT) of 20 patients with MetS without diabetes, ASCVD, smoking or any inflammatory condition, and matched controls. SAT EOS were quantified by immunochemistry using the Vital New Red stain (ABCAM). Both circulating and SAT EOS were significantly increased 2-fold in MetS (p<0.007) and correlated with each other (r=0.40, p=0.03). Circulating EOS correlated significantly (p<0.05) with triglycerides (TG) (r=0.56), HDL-cholesterol (r=-0.45), IL-6 (r=0.57) and chemerin (r=0.61). SAT EOS correlated significantly (p<0.05) with cardio-metabolic factors including glucose (r=0.42), TG (r=0.39), and adipose IR(r=0.64), biomarkers of inflammation, including leptin (r=0.41), IL-6 (r=0.44), adiponectin (r=-0.39), endotoxin (r=0.56), and chemerin, (r=0.57), and SAT markers of fibrosis (collagen (r=0.42) and Sirius red (r=0.44)). In conclusion, we make the novel observation that eosinophils are increased in MetS and that SAT EOS are pro-inflammatory and promote fibrosis in SAT. Hence, they contribute to the dysregulation of SAT biology in MetS. Disclosure K. Moussa: None. P. Gurung: None. S. Devaraj: None. I. Jialal: None.