2098 PROSTATE BIOPSIES FROM AFRICAN-AMERICAN MEN EXPRESS HIGHER LEVELS OF AGGRESSIVE DISEASE BIOMARKERS THAN PROSTATE BIOPSIES FROM CAUCASIAN MEN

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You have accessJournal of UrologyProstate Cancer: Detection and Screening IV1 Apr 20102098 PROSTATE BIOPSIES FROM AFRICAN-AMERICAN MEN EXPRESS HIGHER LEVELS OF AGGRESSIVE DISEASE BIOMARKERS THAN PROSTATE BIOPSIES FROM CAUCASIAN MEN Howard Kim, Daniel M. Moreira, Jayakrishnan Jayachandran, Leah Gerber, Lionel Banez, Robin Vollmer, Amy Lark, Michael J. Donovan, Douglas Powell, Faisal M. Khan, and Stephen J. Freedland Howard KimHoward Kim Durham, NC More articles by this author , Daniel M. MoreiraDaniel M. Moreira Durham, NC More articles by this author , Jayakrishnan JayachandranJayakrishnan Jayachandran Durham, NC More articles by this author , Leah GerberLeah Gerber Durham, NC More articles by this author , Lionel BanezLionel Banez Durham, NC More articles by this author , Robin VollmerRobin Vollmer Durham, NC More articles by this author , Amy LarkAmy Lark Durham, NC More articles by this author , Michael J. DonovanMichael J. Donovan Yonkers, NY More articles by this author , Douglas PowellDouglas Powell Yonkers, NY More articles by this author , Faisal M. KhanFaisal M. Khan Yonkers, NY More articles by this author , and Stephen J. FreedlandStephen J. Freedland Durham, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.2172AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES A wide array of putative biomarkers, including androgen receptor (AR), alpha-methylacyl CoA racemase (AMACR) and Ki-67 are being investigated as predictors of prostate cancer (PCa) diagnosis and recurrence. Given that African-American men (AAM) are at increased risk for PCa progression compared to Caucasian men (CM), we compared the expression of these biomarkers as a function of race among men undergoing radical prostatectomy (RP). METHODS Formalin-fixed, paraffin-embedded prostate needle biopsy specimens from 131 patients treated with RP at the Durham Veteran Affairs Medical Center were H&E stained and immunofluorescent (IF) assayed for AMACR, AR, and Ki67. Proprietary image analysis was used to identify significant IF markers of progression: relative area AR- and AMACR-positive tumor nuclei (AR & AMACR), relative area AR- and AMACR-positive tumor nuclei/lumen area (AR & AMACR/lum), normalized AR intensity in Gleason 1-3 tumor nuclei (norm AR), dynamic AR intensity (dyn AR), relative area Ki67-positive tumor nuclei (Ki67) and relative area Ki67-positive tumor nuclei/lumen area (Ki67/lum). Analysis of baseline characteristics of the study population, stratified by race, was performed using t-test, rank-sum, and x2. The effect of race on expression of IF markers was accomplished using multivariate linear regression, adjusting for age, PSA, year, and biopsy Gleason score. RESULTS Of the 131 patients we identified, 67 (51%) were AAM and 64 (49%) were CM. On analysis of baseline characteristics, all but one IF marker (Ki67/lum, rank-sum, AAM=0.81, CM=0.83, P=0.18) were expressed at higher levels in AAM than CM, with Norm AR (rank-sum, AAM=1.39, CM=1.23, P=0.006) and Ki67 (rank-sum, AAM=0.06, CM=0.05, P=0.02) attaining statistical significance. On multivariate analysis, all IF markers were expressed more in AAM, with Norm AR (AAM=1.100, CM=0.715, P=0.001), Ki67 (AAM=0.051, CM=0.035, P=0.007), and Ki67/lum (AAM=0.589, CM=0.400, P=0.022) being statistically significant. CONCLUSIONS Prostate biopsies from AAM were more likely than those from CM to have higher expression of all adverse biomarkers studied, with Norm AR and the tumor proliferation markers of Ki67 and Ki67/lum reaching statistical significance. These data support the hypothesis that PCa is biologically more aggressive among AAM. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e816 Peer Review Report Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Howard Kim Durham, NC More articles by this author Daniel M. Moreira Durham, NC More articles by this author Jayakrishnan Jayachandran Durham, NC More articles by this author Leah Gerber Durham, NC More articles by this author Lionel Banez Durham, NC More articles by this author Robin Vollmer Durham, NC More articles by this author Amy Lark Durham, NC More articles by this author Michael J. Donovan Yonkers, NY More articles by this author Douglas Powell Yonkers, NY More articles by this author Faisal M. Khan Yonkers, NY More articles by this author Stephen J. Freedland Durham, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...