2091 NOVEL HUMAN AND ANIMAL PROTEIN-FREE SPERM CRYOPRESERVATION MEDIA RESULTS IN POST-THAW SURVIVAL AND RECOVERY EQUIVALENT TO STANDARD FREEZING MEDIA

Abstract

You have accessJournal of UrologyInfertility: Physiology, Pathophysiology, Basic Research1 Apr 20122091 NOVEL HUMAN AND ANIMAL PROTEIN-FREE SPERM CRYOPRESERVATION MEDIA RESULTS IN POST-THAW SURVIVAL AND RECOVERY EQUIVALENT TO STANDARD FREEZING MEDIA Jason Barritt, PhD Joseph Lee, Natan Bar-Chama, MD Boback Berookhim, MD Alan B. Copperman, MD Jay Bertelson, andBS, MT(ASCP) Albert AnounaHCLD Jason BarrittJason Barritt New York, NY More articles by this author , Joseph LeeJoseph Lee New York, NY More articles by this author , Natan Bar-ChamaNatan Bar-Chama New York, NY More articles by this author , Boback BerookhimBoback Berookhim New York, NY, NY More articles by this author , Alan B. CoppermanAlan B. Copperman New York, NY More articles by this author , Jay BertelsonJay Bertelson Mountainside, NJ More articles by this author , and Albert AnounaAlbert Anouna Mountainside, NJ More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.2258AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Optimal sperm survival following cryopreservation and thawing is essential, especially in patients with low sperm counts and donor samples. Sperm cryopreservation media has historically been supplemented with proteins sourced from both humans and/or animals. For patients of some religious beliefs, the inclusion of these proteins can be highly controversial and may inhibit their use of this medical technique. This study tested a novel media free of human and animal proteins, eliminating potential sources of contamination and controversy while maintaining cryoprotective properties. METHODS 15 sperm samples designated for disposal were pooled and washed in Quinn's Fertilization media (Sage) then split for 6 runs using two treatments: standard Sperm Freezing Media (Test Yolk Buffer (TYB), Irvine Scientific) or the experimental Cryolink Freezing Media ((CFM), Biogenetics). Freeze media was added drop-wise and gently mixed for 10 minutes at room temperature. Samples were placed in cryotubes (1ml per tube) and plunged into LN2. Samples were thawed in a 37° water bath for 5 minutes, mixed with wash media drop-wise over 6 minutes. Post-thaw samples were washed, pelleted and resuspended in 0.5ml of wash media. Recovery and survival rates were assessed by count, motility, progression and morphology immediately after thaw and then at 3, 24 and 48 hrs after washing. Data were analyzed by Wilcoxon ranked signs test. RESULTS No significant differences between cryopreservation media were noted in post thaw recovery (71% (TYB) vs. 80% (CFM)), motility (Figure), progression or morphology. CONCLUSIONS Animal and/or human proteins are potential sources of bacterial, viral or prion contamination and their inclusion can be controversial or forbidden for some patients of certain religious beliefs. We tested a novel sperm cryopreservation media prepared free of animal and human proteins and found no difference in sperm survival, recovery, progression or morphology post-thaw over a 48 hour incubation period. With further validation of sperm post-thaw fertilization potential, Cryolink Media could provide a safer and less controversial means for cryopreserving male reproductive tissue. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e843 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jason Barritt New York, NY More articles by this author Joseph Lee New York, NY More articles by this author Natan Bar-Chama New York, NY More articles by this author Boback Berookhim New York, NY, NY More articles by this author Alan B. Copperman New York, NY More articles by this author Jay Bertelson Mountainside, NJ More articles by this author Albert Anouna Mountainside, NJ More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...