208 High-quality fluorescent solvatochromic three-dimensional imaging for dermatopathology with a two-photon excitation laser microscopy

Abstract

Histopathological examination with a sliced tissue stained with hematoxylin-eosin dye (HE) is a fundamental procedure. However, a sliced tissue prepared as the vertical-cross section has only a few micrometers thick; hence several cross-section views need a composite image to see a horizontal cross-section view of a three-dimensional image. A confocal scanning laser microscope or two-photon excitation fluorescent microscopy is suitable for drawing fine pictures of three-dimensional and deep images to obtain a three-dimensional view of the skin tissue.