203 - Evaluation of Serum Redox Homeosthasis of AAS Abusers

Abstract

Introduction The administration of anabolic-androgenic steroids (AAS) for improvement of athletic performance has increased notably during the past three decades, even among non-athletes. Beyond the desired effects for these individuals, these high dosages provoke a series of adverse effect, such as: dyslipidemia, atherosclerosis, hypertension, cardiac hypertrophy, ischemia and acute myocardial infarction, insulin resistance and development of cancer. Interestingly, many AAS-side effects have redox imbalance in their etiology. Objective To evaluate redox homeosthasis in the blood of AAS abusers. Method A total of 30 men who regularly engaged in both resistance and aerobic exercise at fitness academies volunteered for the study (control group: n = 15, age=26.93 ± 1.820 years; AAS group: n = 15, age=30.13 ± 1.762 years). Serum Gluthatione Peroxidase (GPx) and Superoxide Dismutase (SOD) activities were evaluated through Elisa Cayman’s kit, while serum Oxidized Low Density Lipoprotein (OxLDL) was evaluated by enzyme-linked immunosorbent Assay kit (Cloud-Clone Corporation). Testosterone, free testosterone, sex hormone-binding globulin (SHBG), Luteinizing hormone (LH), Follicle-stimulating hormone (FSH) and Estradiol were evaluated by electrochemiluminescence immunoassay (Roche). Data were analyzed by software Graph-Pad Prism 5.0, unpaired t test. Results Serum testosterone (C=579.7 ± 45.16 ng/dL n=15; AAS=989.5 ± 145.5 ng/dL n=15,p=0,0119), free testosterone (C=437.6 ± 33.68 pmol/L n=15; AAS=1104 ± 202.2 pmol/L n=13,p=0,0017) and estradiol (C=27.07 ± 2.634 pg/mL n=15; AAS=92.60 ± 25.46 pg/mL n=15,p=0,0161) were higher in AAS group when compared to C group. Moreover, serum AAS (C=3.267 ± 0.3160 mUl/mL, n=15; AAS= 0.7000 ± 0.2640 mUl/mL n=15,p , LH (C=4.693 ± 0.4076 mUI/mL, n=15; AAS=1.060 ± 0.4985 mUl/mL n=15,p Conclusion Our data shows that redox homeostasis is not affected by AAS abuse.