200 BONE MARROW MESENCHYMAL STROMAL CELL THERAPY FOR RESTORATION OF BLADDER WALL DEFECTS

Abstract

You have accessJournal of UrologyStem Cell Research1 Apr 2012200 BONE MARROW MESENCHYMAL STROMAL CELL THERAPY FOR RESTORATION OF BLADDER WALL DEFECTS Jacques Corcos, Wally Mahfouz, Oleg Loutochin, and Jacques Galipeau Jacques CorcosJacques Corcos Montreal, Canada More articles by this author , Wally MahfouzWally Mahfouz Montreal, Canada More articles by this author , Oleg LoutochinOleg Loutochin Montreal, Canada More articles by this author , and Jacques GalipeauJacques Galipeau Atlanta, GA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.253AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Bladder tissue engineering seems to be a promising avenue for tissue replacement in neurourology and oncology. Our aim is to evaluate the process of bladder regeneration after implantation of bladder acellular matrices (BAMs) seeded with bone marrow derived-mesenchymal stem cells (MSCs) in experimentally damaged bladders. METHODS 72 Sprague-Dawley rats were divided as follows: 6 for harvesting MSCs, 22 for harvesting bladders, and 44 subdivided into 8 subgroups: normal, sham control, partial cystectomy (PC), augmentation with BAMs only and augmentation with MSCs seeded BAMs. At 1 and 6 months, rats underwent cystometrograms before sacrifice, histological analysis and immunohistochemistry after sacrifice with H&E and Masson's trichrome. Urothelial cells were identified by anti-pancytokeratins AE1/AE3 while smooth muscle cells were identified by alpha smooth muscle actin (á-SMA). RESULTS PC group had only 45% of their native bladder capacity at 1 and 6 months. The non-seeded group increased their mean bladder capacities (MBCs) by 55% compared to pre-operative values at 1 month and 65% at 6 months. MSCs-seeded BAMs bladders reached MBCs of 75% and 99% in comparison to original pre-cystectomy volume at 1 and 6 months, respectively. In the MSC-seeded group, organized muscle bundles were present in the grafted patches at 1 month, yet their thickness was attenuated in comparison to normal control bladders. At 6 months, muscle bundles were more prominent with increased thickness. Strong and diffuse á-SMA expression was observed. In non-seeded BAMs, smooth muscles were identified, but in disorganized arrangements and attenuated thickness compared to seeded groups. Multi-layered urothelial regeneration was observed in all groups (anti-pancytokeratins positive). CONCLUSIONS Seeding MSCs onto BAMs is feasible and produce a viable tissue for bladder augmentation. Bladders grafted with MSCs-seeded BAMs showed a more significant increase in capacity and satisfactory muscularis propria development when compared to bladder augmented with BAMs only. Functionality of this new type of biomaterial is still to be proven. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e84 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jacques Corcos Montreal, Canada More articles by this author Wally Mahfouz Montreal, Canada More articles by this author Oleg Loutochin Montreal, Canada More articles by this author Jacques Galipeau Atlanta, GA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...