Abstract
A review taking into account the literature reports covering 20 years of fatty acid analysis by capillary electrophoresis is presented. This paper describes the evolution of fatty acid analysis using different CE modes such as capillary zone electrophoresis, non-aqueous capillary electrophoresis, micellar electrokinetic capillary chromatography and microemulsion electrokinetic chromatography employing different detection systems, such as ultraviolet-visible, capacitively coupled contactless conductivity, laser-induced fluorescence and mass spectrometry. In summary, the present review signals that CE seems to be an interesting analytical separation technique that is very useful for screening analysis or quantification of the usual fatty acids present in different matrices, offering short analysis times and a simple sample preparation step as inherent advantages in comparison with the classical methodology, making it a separation technique that is very attractive for quality control in industry and government agencies.
Highlights
20 Years of Fatty Acid Analysis by Capillary ElectrophoresisMarcone Augusto Leal de Oliveira *, Brenda Lee Simas Porto, Isaura Daniele Leite Faria, Patricia Lopes de Oliveira, Patricia Mendonça de Castro Barra, Renata de Jesus Coelho Castro and Renata Takabayashi Sato
Fatty acids (FAs) comprise carboxylic acids that are aliphatic and typically linear, and monocarboxylic acids with long hydrocarbon chains which are represented by the general formRCOOH, where R represents the carbon chain
FA analysis by capillary electrophoresis (CE) takes into account background electrolyte (BGE) features such as the use of organic solvent in order to avoid micelle formation among the FAs, the fact that the pH must be higher than 7.0 to promote carboxyl group dissociation (FAs have pKa values of about 5.0) and the need to make it possible to analyse FAs in anionic form under counter-electroosmotic flow (EOF) and cathodic EOF, the use of chromophoric species to promote the indirect detection of saturated FAs since they present low molar absorptivity in the UV range and presence of cyclodextrins [32], neutral surfactants or alcohols to improve the selectivity among the cis homologues or cis-trans isomers
Summary
Marcone Augusto Leal de Oliveira *, Brenda Lee Simas Porto, Isaura Daniele Leite Faria, Patricia Lopes de Oliveira, Patricia Mendonça de Castro Barra, Renata de Jesus Coelho Castro and Renata Takabayashi Sato. Grupo de Química Analítica e Quimiometria (GQAQ), Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG 36036-900, Brazil. Received: 5 February 2014; in revised form: 7 August 2014 / Accepted: 25 August 2014 /
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