20 years' experience of quality assurance strategies of a large and ISO 22870 accredited POCT multiparameter network

Abstract

The paper dip-stick immunoassay (DSIA) is rapid, cheap, and easy to produce. As a modified lateral flow immunoassay, DSIA also has obtained significant attention in point-of-care testing (POCT). Investigating more diverse signal carriers for paper immunoassay has always been a research hotspot. In this work, a dual reading (color/catalytic signal) DSIA based on the multifunctional material NiCo2O4 (NCO) is developed for detecting 17β-estradiol (E2). NCO is highly stable and possesses inherent properties of dark black color, nanozyme activity, and magnetism. The colorimetric and catalytic signals-based method of immunoassay strips can obtain a more accurate result maximally. The double reading NCO-DSIA can obtain a limit of detection of 0.2 ng mL−1 and 1 ng mL−1 for E2 under colorimetric and catalysis signals, respectively. In addition, the magnetic property of NCO can contribute to inhibiting the matrix effect of food samples. Furthermore, this non-composite multifunctional nanomaterial-based DSIA has potential development prospects for pollutants detection.