Abstract
Publisher Summary This chapter focuses on preparation of glia maturation factor from bovine brain. The glia maturation factor (GMF) for its profound effect on the phenotypic expression of cultured astroblasts. GMF is known to be a mitogen as well as a maturation agent. GMF reverses some of the aberrant growth properties of glial tumor cells. It also stimulates astrocytes to produce second-order growth factors or hormones. When given to newborn rats, GMF promotes the recovery of brain tissue from injury. GMF appears to be an autoregulatory molecule produced by the brain and capable of controlling cell growth and development in the nervous system. GMF has no species specificity, but bovine brains are used as the source because of high specific activity and economy of cost. An aqueous extract at physiologic pH is first prepared, which is then fractionated with ammonium sulfate precipitation and column chromatography with DEAE-Sephacel, Sephadex G-75, and hydroxylapatite yielding a 10,000 fold partially purified GMF. This preparation is useful for most biological studies.
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