Abstract
Publisher Summary This chapter describes the purification of Escherichia coli α-Hemolysin (AH). The chapter also describes four methods for purification of these AH complexes and point out the advantages and limitations of each. AH lyses erythrocytes from a variety of mammalian species by a mechanism that requires divalent cations. The hemolytic reaction is time and temperature dependent. AH is a virulence factor for many clinical isolates of Escherichia coli . Its role in the pathogenesis of nonintestinal infections is evaluated in a recent review. Investigations into the toxicity of E. coli hemolysin are difficult because of its inability to purify the protein in an active form. Thus, the toxicity of other bacterial products, such as lipopolysaccharide (LPS), in partially purified AH preparations must also be considered in evaluating the importance of the hemolysin in disease. Immunization of animals with AH results in production of antibodies reactive with contaminating E. coli products as well as the hemolytic protein.
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